Interaction of Anticancer Drug Methyl Caffeate with DNA Investigated by Electrochemical and Spectroscopic Methods

摘要

The interaction of methyl caffeate with herring sperm DNA was investigated by cyclic voltammetry, differential pulse voltammetry and ultraviolet absorption spectrometry. Methyl caffeate had a well-defined electrochemical signal in 0.50 M acetate buffer (pH 4.6) involving two electrons and two protons. The binding of methyl caffeate to herring sperm DNA resulted in a series of changes in the electrochemical behavior, giving a considerable decrease in the peak currents and a negative shifts in the peak potential of methyl caffeate, suggesting the interaction of methyl caffeate and herring sperm DNA by an electrostatic mode. Furthermore, the data from the electrochemical investigation showed that a non-electroactive supramolecular compound was formed between methyl caffeate and herring sperm DNA. The conclusion was reinforced by ultraviolet absorption spectrometry.