This work investigated the potential use of benzoic acid (BA)-based immunoadsorbent, a hydrophobic adsorbent, for autoantibodies selective adsorption therapy. The adsorbent was assessed through chromatographer and batch system assays in terms of adsorption capacity, selectivity for both polycloneantibodies in normal human plasma and pathogenic antibodies in patients' sera. For human immunoglobulin G (IgG), capacities of >30 mg/mL at 10% breakthrough (pH 7.4, linear flow rate of 212 cm/hr) were regularly achieved without adding salt. Removal percentage of immunoglobulins (Igs) in plasma samples was 34.2±±4.0%, while that of albumin was less than 3% at the ratio of 5 sample volumes per adsorbent volume. Rheumatoid factors in serum samples from 8 rheumatoid arthritis (RA) patients were eliminated at different degrees after treatment by the adsorbent. The results indicated that BA-based agrose was effective in selectively adsorbing human antibodies from plasma, could thus have a potential ability to eliminate pathogenic autoantibodies in apheresis.
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机译：这项工作研究了基于苯甲酸（BA）的免疫吸附剂（一种疏水性吸附剂）在自身抗体选择性吸附疗法中的潜在用途。通过色谱仪和分批系统分析法评估吸附剂的吸附能力，正常人血浆中多克隆抗体的选择性以及患者血清中的病原性抗体。对于人免疫球蛋白G（IgG），在不添加盐的情况下，通常可以达到在10％穿透（pH 7.4，线性流速为212 cm / hr）时> 30 mg / mL的容量。血浆样品中免疫球蛋白（Igs）的去除率为34.2±±4.0％，而每吸附剂体积为5个样品体积的比率，白蛋白的去除率小于3％。用吸附剂治疗后，在不同程度上消除了8名类风湿关节炎（RA）患者血清样品中的类风湿因子。结果表明，基于BA的琼脂糖可有效地从血浆中选择性吸附人抗体，因此具有消除单采血液中病原性自身抗体的潜在能力。
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