Numerical Simulation of the Horizontal Rotating Bioreactor

摘要

To simulate the internal pressure numerically for the horizontal rotating bioreactor at 15rpm and to study its effect on the cells culture. Using both the theoretical calculation and the FLUENT 6.0 software to calculate and to simulate respectively the pressure inside the bioreactor, we verified experimentally the calculation result by bone marrow-derived mesenchymal stem cells cultured in the bioreactor. At the rate of 15rpm, the inner pressure of the rotating bioreactor obtained by both methds is basically consistent. It is also found that the dynamic pressure ( ) of bioreactor in central area (0