Physical Chemistry Mechanism of Fermentative Hydrogen Production by Mixed Bacteria Culture

摘要

Fermentation Hydrogen producing bacterium is another kind of microbe, which can produce hydrogen in metabolic process. There are a lot of microbes to produce hydrogen through fermentation, such as Clostridium butyricum, Biohydrogenbacterium R3 etc. Physical chemistry mechanism of fermentative hydrogen production is discussed in the paper. There is high hydrogen partial pressure in the anaerobic system of biological hydrogen producing process and integrated oxidation-reduction galvanic couples have been formed, therefore we suppose that oxidation-reduction potential in this system is relevant to the galvanic couple H+/H2, i.e. Ionization equilibrium of organic acid were achieved by organic acid in both system and cell. Carbonic acid equilibrium [HCO3 ˉ/CO2 (aq)] is an important buffer system inside cells for some fermentation bacteria to produce a lot of CO2 except ionization equilibrium of amino acid. CO2 (aq) dissolved in solution and CO2 (g) dissolved in gas phase are at two -phase equilibrium state and form carbonate buffer system (when pH<8, no CO3 2- exists).