Research on Microscopy Automatic Focusing based on Gradient Function

摘要

In the digital imaging system, how to achieve automatic focusing rapidly and efficiently is one of key researches on imaging system. The stepping motor subdivision technique is introduced into automatic focusing system in this paper, and quick large range focusing strategy with combination of coarse tuning and trimming is presented, applying focusing system to high power objective lens of more minimal depth of field, further enlarging the application range of automatic focusing. Finally after experimental verification, the requirements of large range, high precision, quick focusing are met in the system which has wide application.