Aberrant methylation of CpG island stretches within a promoter,causing silencing of tumor suppressor genes,is a widespread phenomenon in cancer cells.Insulin-like growth factor binding protein-7(IGFBP7)gene can modulates growth of embryo and is considered as a tumor suppressor gene.The aim of this study was to develop an oligonucleotide microarray for the detection of the methylation pattern of IGFBP7 gene.In the method,bisulfite-modified DNA was used as a template for PCR amplification.Using this method we successfully detected 15 breast tumor samples and corresponding normal samples and the results were verified by bisulfite cloning sequencing.
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