Study on S180 Tumor Mice Erythrocyte Membrance Function of Sargassum Fusiform Polysaccharides

摘要

Objective To study the effect of Sargassum fusiforme polysaccharides (SFPS) on biochemistry function of erythrocyte membrane of S180 mice.Methods A tumor mouse model was developed,and the mice were treated with high,medium,and low doses of SFPS through abdominal injection for 7 d.Erythrocytes were collected and erythrocyte suspensions were prepared.Changes in [Ca2+] in the erythrocytes in the tumor-bearing mice were measured using laser confocal scanning; changes in the content of sialic acid and the acitivities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase were meausred using relevant test kits; changes in the level of erythrocyte membrane potential were analyzed using flow cytometry; and changes in the complex mobility of erythrocytes were measured using high performance capillary electrophoresis (HPCE).Results SFPS lowered the intracelluar [Ca2+] of erythrocytes in tumor-bearing mice,increased the content of sialic acid on surface of erythrocyte membrane,increased the acitivities of Na+,K+-ATPase and Ca2+,Mg2+-ATPase,and raised the level of erythrocyte membrane potential and the complex mobility of erythrocytes in elelctrophoresis.Conclusions Sargassum fusiforme polysaccharides (SFPS) can adjust or restore some biochemistry functions of erythrocyte membrane of S180 mice.