Technique of plant regeneration from immature inflorescence of Pennisetum Purpureum in Vitro

摘要

Immature inflorescences of Pennisetum Purpureum and MS improved and were used for explant and basic culture medium.Effects of development period of immature inflorescences and hormone compositions of different kinds and ration on callus induction and plant regeneration were studied.Results indicated that 2 ～ 5 cm immature inflorescences was optimum.The frequency of callus of compact, small pellet induction reached separately 79.0 % and 72.6% in the callus induction medium supplemented with 4.0mg/L 2,4‐D ＋ 0.05 mg/L KT and 4.0mg/L 2,4‐D ＋ 0.1 mg/L KT ( Figure 1).During subculture, Callus of small pellet were maintained 40.9% and 74.0% in the callus subculture medium added 3.0mg/L 2,4‐D ＋ 0.2 mg/L 6‐BA.The rate of green plant regeneration of small pellet callus from subcultures reached respectively 36.4% and 38.5% in the differentiation medium supplemented with 2.0mg/L CPPU ＋ 0.01mg/L NAA or 0.5mg/L KT ＋ 0.5mg/L IAA.Green plant of regeneration with three leaves was transferred to root vigor medium added 0.5 mg/L NAA in 1/2 MS basic culture medium (Figure 2).The survivor rate of green plant cultured in soil reached above 95% (Figure 3).It was a simple effective method to overcome the obstruction of plant generation by selecting the callus of dry,compact, small pellet in early generation.