Inheritance and molecular mechanism of the genic male sterility accession 'Shaan-GMS' in Brassica napus L.

摘要

Genic male sterility (GMS) is one of the most important ways to utilize the heterosis in B. napus and the exploitation of the new source of male sterility is always its important basic work. Shaan-GMS was a newly discovered male sterility accession in B.napus in 1994, which fertility was very stably expressed in various genetic backgrounds and environments. In this paper, 83 B. napus accessions from China ad abroad were tested for their maintaining or restoring ability for Shaan-GMS. Among them, two accessions were screened out with the restoring ability for Shaan-GMS. The genetic pattern of fertility restoration of male sterility of Shaan-GMS was also investigated. Homozygous male sterile line 803AB was developed from the progeny of the cross between Shaan-GMS and its restorer 96-803. Near isogenic line 220AB derived from Shaan-GMS was characterized by isozymes,protein and RAPD molecular markers. PCR technique was employed to isolate gene homologous to the MS2Bnap (X99922.1)from two rapeseed dominant digenic male sterile lines, namely 220A (male sterile) and 220B (male fertile), 6A (male sterile) and 6C (male fertile). It can be concluded that Shaan-GMS had the similar maintaining and restoring relationship with DGMS line 6CA from Shanghai Academy of Agricultural Sciences. However, Both DGMS lines had some differences in the molecular level.Shaan-GMS may be a potentially valuable male sterile resource in population improvement and heterosis utilization in B. napus.