Effects of adhE Gene Knock-out on Hydrogen Evolution of Klebsiella oxytoca HP1

摘要

Klebsiella oxytoca HP1 is a potential H2-producing bacterium we screened from a hot spring. In the present study, metabolic engineering strategy was carried out to construct ethanol pathway mutants by homologous recombination, in order to improve hydrogen production yield. Metabolic fluxes of the wild and the mutants were analysed and compared.