Our study has demonstrated that intact DNA molecules were able to be released from electrospun nanofibrous PLGA scaffold in a controlled manner. With an appropriate formulation of Plasmid DNA, triblock copolymer LEL, and PLGA in 94% DMF +6% TE, more than 70% DNA molecules was able to evenly distribute into PLGA fibers. The release of DNA was controlled by PLGA degradation rate which was able to be adjusted by PLA to PGA ratio. The portion of DNA staying outside the fibers was released via diffusion, which was affected by scaffold thickness and porosity. Choosing electrospinning conditions was able to achieve the control of DNA diffusion rate.
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