Toxicity of Pharmaceutical Wastewater on Male Reproductive System of Mus musculus

摘要

This study reports on the toxic effects of 35-days intragastric perfusion of pharmaceutical wastewater on male reproductive system of Mus musculus. Although the body and organ weights were not altered, treatment of mice with pharmaceutical wastewater resulted in a significant depletion in the relative percentage of elongated spermatid (HC), diploid spermatogonia (2C) and S-phase cells. These alterations in different germ cell populations are reflected in the various germ cell ratios. The 1C:4C and HC:2C ratios showed a significant decline after pharmaceutical wastewater treatment, while the 4C:2C and 1C:2C ratios increased significantly. Statistically significant sperm abnormalities revealed the genotoxic potency of this pharmaceutical wastewater. Testicular histopathological studies of treated animals revealed expansion of interstitial space and reduction in the number and size of Leydig cells. Thus, the present study has established toxicity of pharmaceutical wastewater on reproductive biology of male mice.