Investigation of the relationship of radio-induced apoptosis of Drosophila larvae nervous system and the ageing of imago nervous system

摘要

The analysis of age-dependent change of nervous system activity of Drosophila melanogaster imago (with use of the test on negative geotaxis) at wild type strain Berlin and strains with mutations of apoptosis genes апоптоза reaper, grim, hid, th~1, th~4, Dcp-1~(02132), Dcp-1~(k05606) and dArk~(k11502) is carried out. It was used Drosophila individuals, developing in conditions of a chronic low dose irradiation. Besides was studied an apoptosis level in nervous ganglion of third instar larvae after irradiation and in the control with use of hardware-software complex DiaMorph Cito (Moscow). The exposition doze was 0.17 sGy/h. The absorbed doze for one generation (from an embrio stage up to imago start, 10-12 days) corresponded 60 sGy. It was shown, that the irradiation of strains with the increased apoptosis sensitivity results in elevated nervous - muscular activity of imago during all experiment periods. At th~1 strain increase of activity in comparison with the control in the first week has made 41%, and in two subsequent - about 80%. Last week authentic increase did not observe. At th~4 strain statistically significant increase of activity in comparison with the control observed in the first week of experiment (18%), in the second (67%) and the fourth (88%). The irradiation of a wild type strain Berlin has caused authentic increase of activity in comparison with the control for the thirteenth day (on 17%) and for the twentieth day (37%). At the same time, at strains with the lowered sensitivity to apoptosis: at strain which individuals are heterozygous in deletion of proapoptosis genes rpr, grim and hid; at strains with dysfunction of proapoptosis gene dArk; at strains with dysfunction of caspase Dcp-1 (Dcp-1~(02132) and Dcp-1~(k05606) allels) authentic changes of nervous - muscular activity it was not shown. Exception has made action of an irradiation on strain with rpr deletion, at which activity only in the first week after influence increased on 39%.