Dielectrophoresis (DEP) of Cells and Microparticle in PDMS Microfluidic Channels

摘要

Electromanipulation of microparticles utilizing microelectrodes has demonstrated considerable promise for the characterization, separation and handling of biological cells. Presently considerable research and development effort is directed towards the development of miniaturized fluidic systems with integrated dielectrophoresis (DEP) electrodes. The design, development and fabrication of a DEP microfluidic assembly with an in-built interdigitated microelectrode array is presented. Continuous fractionation of microparticles in a PDMS microfluidic channel is described. Experimental verification of positive and negative DEP of yeast cells and polystyrene latex beads is demonstrated.