Found of a Technique Detecting SARS Virus RNA and It's Primary Application on Samples from SARS Patients

摘要

Objective To found a technique detecting SARS virus RNA with a good sensitivity and specificity beneficial to the early diagnosis and distinguish diagnosis of the SARS (Severe Acute Respiratory Syndrome) . Methods Collecting samples from 20 cases of SARS patients and 5 non - SARS individuals. Isolating total RNA from the samples and reverse transcript the RNA to the 1st chain of the cDNA. A 140bp fragment of SARS genome was amplified by PCR using a pair of primers, which were designed by ourselves. The PCR products were analyzed by 2% agarose gel electrophoresis followed by EB staining. Results PCR conditions were optimized to obtain a good specificity and good sensitivity,and used to detect the all samples.The 140bp band was found in 6 urine samples among 11 samples, in 3 stool samples among 8 samples, in 6 nasal swabs among 15 samples, in 5 among 15 throat swabs samples, in 2 among 15 serum samples and in 2 among 5 WBC samples. Among the 20 cases of SARS patients, each of 15 cases has one to four samples detected as SARS virus - positive. Conclusion The founded technique can be used to detect the SARS virus RNA in samples to determine whether the patients were infected with the SARS virus or not. Urine and stool are good source for the detection of the SARS virus with a potential high positive rate. It is beneficial for higher detection positive rate to collect more categorized samples from every patient.