Vitrification fulfills its promise as an approach to reducing freeze-induced in jury in a multicellular tissue

摘要

The destructive effect of extracellular ice formation in organized multicellular tissues is known to be a principal reason why conventioonal techniques of cryopreservation fail to provide effective protection during freezing. Avoidance of ice during cooling can physically be achieved by vitrification, which is the amorphous solidification of a supercooled liquid by adjusting the solute composition and cooling rate such that nucleation and growth of ice crystals is prevented.