Development of an ultrahigh-throughput screening system: plate design liquid handling and image analysis

摘要

Abstract: Recent advances in plate design, liquid handling, and imaging have made it possible, for the first time, to create truly ultra-high throughput screening systems. The key engineering challenges which needed to be overcome in order to develop this system as well as system limitations are discussed. Data from model systems is presented comparing the results obtained from the miniaturized format with those obtained from the standard 96-well format. These results demonstrate that the signal to noise ratio and robustness of an assay can be preserved upon ultra-miniaturization. The flexibility of this system should make it amenable for screening of not only pharmaceuticals but also for agrochemicals and applied materials.!9