Laser trapping microscopy as a diagnostic technique for the study of cellular resopnse and laser-cell interactions

摘要

Abstract: Optical laser trapping microscopy has emerged as apowerful tool not only for the optical manipulation ofcells and macromolecules, but also for the study ofcellular physiological responses via force transductionand fluorescence imaging. We describe here the mostrecent results from our laboratory in the use andapplication of laser trapping microscopy to a varietyof studies at the cellular and molecular levels.Fluorescence spectroscopy and imaging have beensuccessfully combined with optical micromanipulation. Asingle near-infrared laser beam is used for two-photonfluorescence excitation and micromanipulation oftrapped biological specimens. Cell viability isobserved and monitored with a Nd:YAG laser ($lambda $EQ1064 nm) and an Al:GaAs diode laser ($lambda $EQ 809nm). Traps and conventional fluorescence imaging arealso used simultaneously to examine T-cell activationdynamics.!7