In-vivo interleukin-1-alpha induction following chlorin-e6 photodynamic therapy in Balb/c mice

摘要

Abstract: Photodynamic therapy may induce in in-vivo conditions the cytokine Interleukin-1-alpha in Balb/c mice. The sensitizer, i.e. chlorin e$-6$/, in the doses 2.5 and 5.0 mg/kg of body weight followed by light treatment with doses 50 and 100 J/sq.cm resulted in the increase in serum levels of the cytokine. The levels of Interleukin-1-alpha have been determined at different time points using enzyme-linked immunosorbent assay (ELISA). These levels in control animals did not exceed the mean value of 15 pg/ml, whereas in photodynamically treated mice the levels were almost 3 - 4 times higher. The entire experiment has been carried out on healthy animals. !14