Subcellular photodynamic action sites of sulfonated aluminum phthalocyanines in a human melanoma cell line

摘要

Abstract: By means of scanning and transmission electron microscopy the subcellular target sites of photodynamic therapy (PDT) with derivatives of sulfonated aluminum phthalocyanine (AlPcS$-1$/, AlPcS$-2$/, AlPcS$-3$/, and AlPcS$-4$/) were studied in a human melanoma LOX cell line. It was found that PDT with AlPcS$-1$/ or AlPcS$-2$/ damaged mainly the biomembraneous system of the cells, such as cytoplasmic membrane, mitochondria, endoplasmic reticulum, etc., while AlPcS$-3$/- or AlPcS$-4$/- induced PDT largely destroyed the lysosomes. However, none of the AlPcS$-n$/s led to nuclear damage at an early stage after PDT. The subcellular photodynamic targets of the derivatives of AlPcS$-n$/ are related to the subcellular localization pattern of the dye in the LOX cells. !21