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Imaging of scattering media by phase-correlated single photon counting (PCSPC)

机译:通过相位相关的单光子计数(PCSPC)对散射介质进行成像

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Abstract: Investigations in performing an optical tomography of biological specimen have been carried out by using the phase-correlated single photon counting method. This method is capable of selecting non-interacted, on-beam-axis forward scattered and snake-line photons, i.e. the straight-forward propagating photons. The phase correlated single photon counting unit performs the mutual monitoring of a high-speed photomultiplier tube and adjacent gain by a broadband preamplifier. The detection occurs in phase correlation to the difference frequency of the optical modulation of the two arms of a Mach-Zehender interferometer arrangement. The object arm is crossing the scattering test medium. To prevent strong intensity ratios between the two interferometer arms the reference arm crosses an optically equivalent phantom medium, but without introduction of inhomogeneities. The correlation between the phase of the optical modulation and the electrically derived phase can be varied by 2$pi phase shifting of the electrical signal. This allows the user to set a phase range comparable to the time window in time-resolved detection. Two different principle methods can be used for generating tomographic images: the confocal scanning and conventional (object rotation and translation) tomographic setups. !9
机译:摘要:已经通过使用相位相关的单光子计数方法进行了生物标本的光学层析成像的研究。该方法能够选择非相互作用的,在光束轴上的向前散射和蛇线光子,即,正向传播的光子。相位相关单光子计数单元通过宽带前置放大器对高速光电倍增管和相邻增益进行相互监视。该检测与Mach-Zehender干涉仪装置的两个臂的光学调制的差频成相位相关。物镜臂越过散射测试介质。为了防止两个干涉仪臂之间的强强度比,参考臂穿过光学等效的幻像介质,但不引入不均匀性。可以通过电信号的2πpi相移来改变光调制的相位与电导出的相位之间的相关性。这使用户可以在时间分辨检测中设置与时间窗口相当的相位范围。可以使用两种不同的原理方法来生成断层图像:共聚焦扫描和常规(对象旋转和平移)断层扫描设置。 !9

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