Abstract: Two methods are proposed to improve microscopic fluorescence images. In the first method, the second derivative of the image is taken with respect to the focal depth. To obtain the derivative, the focus of the microscope objective is modulated piezoelectrically by 5 - 10 micrometer, and three images with focal positions z plus h, z and z minus h are taken. The resulting image is calculated according to the formula h$+2$/ f'(z) $APEQ 2f(z)-f(z plus h)- f(z minus h) that follows immediately from Taylors formula. In the second method, a 2D- Fourier transform is applied to the image, followed by multiplication with a model function that reduces low spatial frequencies. Then, the image is transformed back into the spatial domain. The improvements are demonstrated on fluorescence microscopical images of bronchial tumor tissue. !11
展开▼
机译：摘要：提出了两种方法来改善微观荧光图像。在第一种方法中，相对于焦深，拍摄图像的第二导数。为了获得衍生物，显微镜物镜的焦点通过5-10μm调制5-10微米，并采取具有焦点位置Z加H，Z和Z减去H的三个图像。由所公式的H $ + 2 $ / f'（z）$ apeq 2f（z）-f（z plus h）-f（z minus h）计算所得到的图像，从Taylors公式中立即进行。在第二种方法中，将2D傅里叶变换应用于图像，然后乘以模型函数，其减少低空间频率。然后，将图像转换回空间域。在支气管肿瘤组织的荧光显微图像上证明了改进。 ！11
展开▼
