Innate tissue fluorescence of the oral mucosa of controls and head-and-neck cancer patients

摘要

Abstract: Base line spectral excitation and emission scans were defined for the oral mucosa in a population of 61 controls, 16 oral tongue cancer patients and 2 patients with tongue leukoplakia. A xenon-based fluorescence spectrophotometer (Mediscience Corp.) with a fiberoptic probe (Mediscience Corp.) was used to collect excitation and emission spectra. Two excitation scans ($lambda@Ex 200-360 nm, $lambda@Em 380 nm; $lambda@Ex 240-430 nm, $lambda@Em 450 nm) and two emission scans ($lambda@Ex 300 nm, $lambda@Em 320-580 nm; $lambda@Ex 340 nm, $lambda@Em 360-660 nm) were used to analyze the buccal mucosa (BM), hard palate (HP), floor of mouth (FOM) and dorsal tongue (DT) of 61 control individuals. In 41 controls the lateral tongue site (LT) was added. The same set of scans was performed on tumor lesions and contralateral normal tissues of 16 patients with lateral tongue tumors and on two individuals with leukoplakia of the tongue. Ratios of points on the individual scans were used to quantitate data. The excitation scan ($lambda@Ex 200-360 nm, $lambda@Em 380 nm) and the emission scan ($lambda@Ex 300 nm, $lambda@Em 320-580 nm) were able to statistically discriminate the HP and DT from the BM and FOM. The ratios of intensities of neoplastic mucosa and contralateral sites were significantly different with the excitation scans ($lambda@Ex 200-360 nm, $lambda@Em 380 nm, p $LS 0.001) and ($lambda@Ex 240-430 nm, $lambda@Em 450 nm, p $LS 0.01) and with the emission scan ($lambda@Ex 300 nm, $lambda@Em 320-580 nm, p $LS 0.001). Discrimination was significant with the emission scan ($lambda@Ex 340 nm, $lambda@Em 360- 660 nm, p $LS 0.07). Innate tissue fluorescence has potential as a monitor of cancer patients and populations at risk for head and neck cancer.!24