Photodynamic effect on specific antitumor immune activity

摘要

Abstract: In this study the effect of PDT on the antitumoral specific immunologic response was evaluated. We compared the specific cytolytic activity (CLA) by a chromium release assay of primed mouse spleen T lymphocytes sensitized against syngeneic mastocytoma P511 cells. P511 cells, or lymphocytes, or both cells were treated or not with photofrin and/or light (514 nm). Photofrin II alone (1 $mu@g/ml, 2 hours) reduced CLA 59% when P511 were treated. Photofrin II (1 $mu@g/ml) followed by light (25 Joules/sq cm) also reduced CLA 35%. Photofrin II alone (0.5 $mu@g/ml, 2 hours) reduced CLA 8% when only lymphocytes were treated. And Photofrin II (0.5 $mu@g/ml) followed by light (25 Joules/sq cm) also reduced CLA 45%. When both cells were treated with Photofrin II alone or followed by light (25 Joules/sq cm) the CLA was also reduced respectively 19, 41%. !7