Participation of some radical products in isolated nerve cell response to blue laser microirradiation

摘要

Abstract: For the study of some primary photochemical mechanisms of neuron response to laser microirradiation it was modified with quenchers of intermediate photochemical products. Dibunol (0.02 mM), inhibitor of lipid peroxidation, did not shift impulse activity itself, but changed neuron response to microirradiation: impulse frequency acceleration was lower than control, spike generation ceased at higher frequency levels, and neuron lifetime increased by 1.7 times. Hence, impulse acceleration phase is probably connected with membrane lipid peroxidation induced by blue light and dibunol protects cells from laser injury. The study of hydroxyl radicals (important in ionizing radiation cell damage) participation using quencher D- mannite (10 mM) did not show reliable changes of neuron response parameters, i.e., these radicals were not significant for blue laser light induced neuron activity changes. Observation of flavin-like action spectrum with a maximum near 460 nm forced us to use a flavin triplet quencher. Ten mM NaJ did not shift impulse frequency level but changed neuron response to irradiation: cell lifetime increased by 1.4 times due to inhibition phase increase. It is not proved that flavin triplets were involved in neuron response and its lifetime increase was due to rather physiological than photochemical causes. !6