Characterizing Electrogenic Capabilities of Human Gut Microbes

摘要

We report a low cost, disposable, paper-based sensing platform for rapid identification and characterization of electroactive microorganisms in human gut. 21 spatially distinct wells of a sensor array parallely characterize microbial extracellular electron transfers of five gut microbes (Staphylococcus aureus, Enterococcus faecalis, Streptococcus agalactiae, Lactobacillus reuteri and Lactobacillus rhamnosus) in a defined, microliter culture volume by directly measuring microbial electricity generation in a novel microbial fuel cell configuration. S. aureus, E. faecalis and S. agalactiae showed distinct electrogenic capabilities. The electrogenic potential of gut microbes will be harnessed to act as a biocatalyst and produce a sufficient electrical current for practical applications.