Fast tissue investigation using label-free point- and angle-scanning widefield multiphoton microscopies

摘要

Multiphoton microscopy has recently emerged as a powerful high-resolution and label-free imaging modality to study biological specimens in-vivo. Multiphoton modalities require minimal sample preparation showing potential in applications benefitting from fast imaging protocols, such as in medical diagnosis. Here, we overview our recent work on this topic focusing on technique development that could provide fast imaging capabilities to rival conventional imaging modalities. In particular, we demonstrate a novel widefield multiphoton technique that greatly facilitates wavelength-scanning multimodal imaging.