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Fast tissue investigation using label-free point- and angle-scanning widefield multiphoton microscopies

机译:使用无标记点和角度扫描宽场多光子显微术进行快速组织检查

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Multiphoton microscopy has recently emerged as a powerful high-resolution and label-free imaging modality to study biological specimens in-vivo. Multiphoton modalities require minimal sample preparation showing potential in applications benefitting from fast imaging protocols, such as in medical diagnosis. Here, we overview our recent work on this topic focusing on technique development that could provide fast imaging capabilities to rival conventional imaging modalities. In particular, we demonstrate a novel widefield multiphoton technique that greatly facilitates wavelength-scanning multimodal imaging.
机译:最近,多光子显微镜已成为一种强大的高分辨率且无标记的成像方式,可以在体内研究生物标本。多光子模态需要最少的样品前处理,从而在受益于快速成像方案的应用中显示出潜力,例如在医学诊断中。在这里,我们概述了我们在该主题上的最新工作,重点是可以提供快速成像功能以与传统成像方式相媲美的技术开发。特别是,我们展示了一种新颖的宽场多光子技术,该技术极大地促进了波长扫描多峰成像。

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