We have developed a novel tumor mimetic microfluidic chip with an intricate vascular design that can be used for demonstrating a high degree physiological relevance of the native breast cancer TME. This model also facilitates understanding of complex tumor heterogeneity in an in vitro platform with real-time visualization capabilities of cell migration and the potential to assess anti-cancer drug efficacy. This study established a novel cancer-on-a chip platform for the investigation of long-term coculture. Finally, testing of anticancer drug efficacy and cytotoxicity revealed a prominent difference in drug action based on the geometry and design of microfluidic chips. Overall, this cancer-on-a-chip platform can be used in the future for testing a larger numbers of potential drug candidates and for detailed investigation of various tumorigenic mechanisms related to EMT, metastasis, and tumor-related angiogenesis.
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