Pumpless platform for high-throughput dynamic multicellular culture and chemosensitivity evaluation

摘要

The culture platform was designed, fabricated, and evaluated to: (1) control and maintain flow rate within 10% under the gravity-based pumpless configuration, necessary for osteoblastic stimulation while eliminating potential sources for leaking and bubble formation and (2) demonstrate high-throughput in situ cell staining and imaging using HCS for drug response evaluation of multicellular cultures.