Apoptosis-Mediated Decellularization Approaches for Lung Tissue Engineering

摘要

Lung pieces were successfully decellularized by inducing apoptotic death of tissue-resident cells via chemotherapeutic drug camptothecin and FasL. Light detergent wash improved cellular component removal without compromising tissue architecture. Future directions include digesting decellularized lung pieces in acid-enzyme solution to create hydrogels. Subsequently, these hydrogels will be recellularized with vascular and airway cells to recapitulate the healthy and pathological microenvironment of lung before and after COPD.