Effect of Glycoproteins Extracted from Perna Viridis (GPP) on Immunologic Function in Immunosuppressive Mice

摘要

The effect of GPP, glycoproteins extracted from P.viridis, on regulation of immune function in cyclophosphamide (CTX)-induced immunosuppressive mice was evaluated. Different dosages of GPP (50, 100, 200 mg/kg) were given as the low, middle, and high group mice. The group injected with CTX was adopted as model group, with the group without treatment as the control group. The index of immune organs such as liver, spleen and so on, the transformation and propagation ratio of T lymphocyte, the activity of NK cell, the humoral immunity, macrophage phagocytosis, the level of cell cytokines and hemolysin were all assessed. The experiment results revealed that GPP could significantly promote the proliferation of splenic lymphocytes, increase the ratio of CD4+T and CD8+T lymphocytes, and enhance the activities of NK cells and cytokines in a dose-dependent manner in CTX-induced immunosuppressed mice. Furthermore, GPP boosted the level of hemolysin and the ability to engulf neutral red, expurgatory index k, and phagocytosis index α (P<0.05). Therefore, GPP may play an important regulatory role in immune responses by enhancing cellular, humoral and innate immunity, consistent with possible anti-oxidative function.