Liquid Chromatograph - Mass Spectrophotometer and Anti Uric Acid Potential Studies of Ethyl Acetate Extract of Archidendron bubalinum (Jack) I.C. Nielsen Fruit Seed Shell

摘要

This study focused on identifying the types of compounds contained in the ethyl acetate extract of seed shell of Archidendron bubalinum (Jack) I.C. Nielsen (AbJICN) from Lampung Indonesia using Liquid Chromatograph Mass Spectrophotometer (LC-MS) and determining the potential of anti-uric acid in the identified compounds. The experiment consists of four stages, which are 1) extraction the fruit seed shell of AbJICN using ethyl acetate, 2) phytochemical test, 3) determination of chemical structure using LC-MS and 4) anti uric acid test in vitro by Tri Bromo Hidroksibenzoid Acid (TBHBA) method. The result of phytochemical test found that ethyl acetate extract in fruit seed shell of AbJICN contains compound of flavanoid and terpenoid group. This founding was also supported by the characterization of chemical compounds using LC-MS which indicated the presence of candidates allopurinol and 2 4 7-Trinitrofluorenone compounds. The decreased uric acid by ethyl acetate extract of fruit seed shell was 1.25 mg/dL half times compared to standard allopurinol 2,43 mg/dL. It can be concluded that allopurinol compounds contained in the ethyl acetate extract of seedshell of Archidendron bubalinum (Jack) I.C. Nielsen potentially became an active anti uric acid substance.