Evaluation of Two Different Neutralization Methods for Chitosan Coatings

摘要

These results suggest that the PB neutralization resulted in better coating swelling, adhesion, protein elution. and cell attachment than typical NaOH treatment. This may be rationalized by the fact that NaOH neutralization causes deprotonation of chitosan amines which results in coatings being less swellable and since high pH may cause cleavage of silane bonds, a decrease in coating adhesion strength. It is speculated that PB does not deprotonate chitosan amines resulting in increased swelling and extended protein release which is favorable for growth factor incorporation and delivery. However, it is noted many osteogenic growth factors require longer release for osseointegration. The neutral pH also had less effect on silane bonding resulting in improved coatings adhesion which is desirable for implant applications. Cell adhesion was favorable for both coating groups as indicated by their hydrophilic qualities. Increased cell attachment on PB treated coatings may reflect the way serum proteins from culture medium absorb on coatings via protonated amines. Further in vitro and in vivo analyses are needed to support these results.