Distribution of protoporphyrin IX (PPIX) induced by aminolevulinic acid (5-ALA) in the skin and liver of rats

摘要

Photodynamic diagnosis by fluorescence emission from PPIX induced by 5-ALA can be done with minimal damage to cells using excitation by low power blue light. This technique uses the fluorescence signal emitted by the PPIX to obtain information about its concentration and dynamics without cell damage. The intracellular peak concentration of PPIX has a distinct time dependence for each organ. We have measured the accumulation time for maximum PPIX concentration in Wistar rats (liver and skin) detecting the induced fluorescence in each organ. PPIX accumulation time varies depending on the tissue type. The live shows a higher PPIX concentration. The detection system can measure endogenous PPIX. This fact opens the possibility of in-vivo displastic tissue diagnosis since these tissues have significant endogenous PPIX concentration.