Development and validation of a method for 11-nor- 9-carboxy- delta9- tetrahydrocannabinol quantification using gas chromatography coupled to triple quadrupole mass spectrometry

摘要

This paper presents a method for 11-nor-9-carboxy-delta9-tetrahydrocanabinol (THC-COOH) quantification from urine samples, for doping control purposes. The method involves gas chromatography coupled to triple quadrupole mass spectrometry (GC-MS/MS). Considering the complexity of the urine matrix, this technique allows for an improved selectivity, compared to the previously used gas chromatography - single quadrupole mass spectrometry (GC-MS) method. World Anti-Doping Agency (WADA) Technical Documents are imposing a threshold of 150ng/mL, a maximum combined standard uncertainty at threshold of 10% and calibration range between 50%-200% of the threshold. The method developed has been proved to be fit for purpose, with a combined standard uncertainty at the threshold of 7.61%.