Biological Control of Post-Harvest Banana Diseases Using Antagonistic Bacteria in Thailand

摘要

Gros Michel banana is an important economically tropical fruit. It is easy to grow and highly nutritious with increasing demand in the market. This research was conducted to study the type of microorganisms that cause of Gros Michel banana disease in Thailand and determine inhibition rate of post-harvest banana diseases using antagonistic bacteria. The study also includes methods to prevent diseases and prolong the shelf life of Gros Michel banana by using antagonist microorganism. The results showed that Curvularia sp., Nigrospora sp., Fusarium sp. and Colletotrichum circinasns (Berk.) Voglino were found in Thai Gros Michel banana farm. However, the Percentage Inhibition of Radial Growth (%PIRG), UN1 has the highest inhibition efficiency of Curvularia sp. (80%), followed by B23 that inhibited at 66.67% of Fusarium sp. and the highest % PIRG at 3 mL (91.60%) tested by dual culture method. The results showed that B23 and UN1 were able to inhibit growth of Curvularia sp. more than 85% of PIRG at 2 mL and 3 mL respectively. It can reduce the use of chemicals for more than 250 ppm. Analysis Identify the type of microorganisms by biochemical characteristics using API found that B23 and UN1 were similar to those of Bacillus subtilis/amyloliquefaciens at 96.6% and 99.6% respectively. The study of biological control from this research can be further developed in banana field in order to reduce the use of chemical, develop disease prevention method, and extend the shelf life of Gros Michel banana by using antagonist bacteria.