Construction of Tip60-Encoding Plasmid and the Effect of Tip60 on the Expression of HPV18 Genes in HeLa Cells

摘要

Tip60 is a cellular protein belonging to the MYST family of histone acetyl transferases (HATs) and it is universally expressed in various types of cells. Tip60 was originally isolated as an HIV Tat-interactive protein with molecular weight of 60 kDa thus was named Tip60 [1]. Tip60 functions as a transcriptional co-factor in the regulation of HTV gene expression. Later on, the histone acetyl transferase (HAT) activity of Tip60 was identified [2]. In addition to histone, Tip60 was also found to be a lysine acetyl transferase therefore it is also known as KAT5. ER, a well-known transcription activator, is a typical target of Tip60 for acetylation [3].