Conclusions: Here we report the very high replicate and high-throughput analysis of cell-biomaterial interactions. Unlike other systems, a large number of individual replicates are analyzed to represent population-level response to biomaterials and results are not skewed by a high-performing subpopulation. Using a 4-plex model biomaterial system, the osteogenic differentiation of hMSCs in collagen and hyaluronic acid-containing microhydrogels was tested. The demonstrated system has the potential to expand to > 300 biomaterials using current technologies. As imaging flow cytometers become increasingly common, this method can collect biomaterials data with high statistical power for increased reproducibility and could in future apply to functionality-based sorting of biomaterial-encapsulated cells.
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