Conclusions: This research model provides an optimized empirical cryopreservation process that allows for extended use with maximum cell viability. Upon completion it will produce a comprehensive standard protocol for cryofreezing engineered hepatic tissue. This model can be expanded more broadly into methodology for extending all engineered tissues' shelf life in order to maximize preservation and use. The by product is an ex-vivo liver model whose efficacy can then be evaluated for larger application in drug toxicity testing.
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