Identification of Sulfur Activation Relevant Protein Genes of Extremely Thermophilic Acidianus manzaensis

摘要

The sulfur activation by extracellular proteins is considered as the crucial stage during biooxidation of elemental sulfur (S°). In order to study genes encoding sulfur-activation related extracellular proteins of extremely thermophilic Acidianus manzaensis, the extracellular proteins with higher abundance for the strain grown on S° allotropes than that on Fe~(2+) were first screened by two-dimensional gel electrophoresis, and then identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Nine genes amplified with PCR were satisfactory according to their agarose gel electrophoresis. The differential expression of these nine genes when the strain grown on S° allotropes and Fe~(2+) were analyzed with real-time quantitative PCR (RT-qPCR). Results showed that seven of them were higher expressed when the strain grown on S° allotropes than on Fe~(2+), indicating they may be related with sulfur activation by A. manzaensis.