Effect of Mechanical Property of Scaffold Surface with Micro Hybrid Striped Pattern on Cell Migration

摘要

The effect of the mechanical property of the surface of the scaffold with the micro hybrid striped pattern on the migration of cells has been studied in vitro. The surface of the scaffold with the micro stripe pattern of bands (the width around 0.1 mm) was made by the photolithography technique. The variation of hardness of the surface of the band has been made of the materials alternately: polydimethylsiloxane (PDMS), and epoxy based negative photoresist material (SU-8). Three kinds of cells were used in the experiment: Neuro-2a (a mouse neural crest-derived cell line), L929 (fivroblast connective tissue of C3H mouse), and C2C12 (mouse myoblast). Cells were seeded on the micro pattern, and incubated for 24 hours in the medium. The total areas of cells at each band were evaluated at the microscopic images. The experimental result shows that most of cells migrate to the area of the band with SU-8, and that the tendency is considerable on L929. The experimental system is effective to investigate durotaxis of cells.