Combined Video Analysis of ICG and 5-ALA Induced Protoporphyrin IX and Hemoglobin Oxygen Saturation in near Infrared

摘要

Due to the high recurrence rate after the glial brain tumor removal, methods of intraoperative navigation have a high relevance, providing the most complete removal of tumor tissues with maximum preservation of healthy ones. In this work a combined visualization method is proposed with an assessment of fluorescence and diffuse reflectance images. Fluorescence intensity of 5-ALA-induced protoporphyrin IX allows visualization of tumor cells, distribution of indocyanine green fluorescence helps to visualize the vascular system of the tumor, and parallel mapping of the degree of oxygenation demonstrate the hypoxic regions. The images were obtained in the near infrared range of the optical spectrum in order to maximize the optical probing depth in the window of biological transparency.