Polymersomes: Towards Treatment of Neurodegenerative Disorders through Enzyme Replacement Therapy

摘要

Incorporation of both mannitol and inulin was confirmed using Fourier transform- infrared spectroscopy, with absorbance increasing with increasing molecule concentration. The PSDs of polymersomes were not negatively affected by the incorporation of molecules, with no statistically larger diameters found over a period of 2.5 hours when compared to empty polymersomes. At 120 minutes, the incorporation of mannitol at both 2 wt%/v and 8 wt%/v lead to polymersome diameters statistically smaller than empty polymersomes at the same time point. Karl Fischer titration showed that increasing inulin concentration decreased the moisture content found after lyophilization, which is beneficial to encapsulation of enzymes, whose activities are vastly affected with crystallization. The introduction of these molecules led to reproducible effects with respect to size control, allowing for the best opportunity to cross the BBB.