Intra-lymph node delivery of biomaterial depots prevents and reverses Experimental Autoimmune Encephalomyelitis

摘要

A single dose of MOG/Rapa depots, injected directly to the inguinal LNs of mice, was able to halt or reverse EAE. Ongoing studies aim to identify the mechanism of action, including testing depot effect on DC activation and T cell phenotype (e.g., T_(REGS), T_H17) in vitro. Future in vivo studies will test immune cell frequency and phenotype, particularly in the LN microenvironment, via FACS and immunohistochemistry. Finally, we aim to test the antigen specificity of this tolerogenic effect to evaluate the potential to control autoimmunity without broad immunocompromise.