The cloning and analysis of a partial lipase gene sequence of Staphylococcus hominis GIMT1.079 - Subtitle: Partial lipase gene sequencing of S. hominis

摘要

Lipase activity is common in staphylococci. Based on the blast of various known staphylococcal lipase DNA sequences, the degenerate primers were derived to amplify a 782-bp consensus lipase gene sequence of Staphylococcus hominis GIMT1.079, which encoded a deduced polypeptide of 260 amino acid (aa) residues. The alignment of aa sequence revealed that this 260-aa partial lipase of S. hominis shared high homology with those conserved parts of other 11 deduced staphylococcal lipases, in the range of the lowest 43.0% of S. epidermidis and the highest 63.5% of S. saprophyticus. Two aa residues, Ser~(39) and Asp~(230), were preliminarily confirmed in the putative catalytic triad as well as the 'P-loop' motif (-[AG]-x4-G-K-[ST]-) in this deduced 260-aa partial S. hominis lipase sequence.