Aerobic Denitrification of Nitrate Wastewater and dynamic analysis of the Microbial Community in a Bio-ceramic Reactor

摘要

Biological treatment of nitrate wastewater with aerobic denitrifiers in a bio-ceramic reactor was investigated. And denaturing gradient gel electrophoresis (DGGE) method was used to analyze the stability of microbial community structure. With the influent hydraulic loading 0.75m/h, gas/water ratio 5～ 10:1, temperature 15 ～ 23°C, COD loading rate 1.92 ～ 5.98kg·m~(-3) · d~(-1) and (NO_3)~--N loading rate 0.60～1.34 kg·m~(-3) · d~(-1), high nitrate nitrogen removal was almost achieved 100% and the maximum total nitrogen removal was reached up to 95.73% during stable operation. In addition, the nitrite concentration of effluent was lower. The PCR-DGGE profiles showed that shift of microbial diversity correspond to the effect of nitrate removal and the preponderant populations for aerobic denitrification were steady during the test.