Using SPIM to track the development of the focal power of the zebrafish lens

摘要

Selective plane illumination microscopy (SPIM) is a 3D imaging technique that uses a sheet of light to optically section a sample in vivo. A cylindrical lens focuses collimated light in one dimension, producing a sheet that is formed in the sample via an objective lens. Any optical power within the sample will additionally refract the light sheet passing through it. We exploit this effect to track the development of the optical power of the zebrafish lens over the first 4 days post fertilisation (dpf). We show that light is focussed on to the photoreceptor layer of the retina at 4 dpf.