Molecular Cloning and Characterization of Glycerol Dehydrogenase from Klebsiella pneumoniae

摘要

Glycerol dehydrogenase (GDH) was a key enzyme for 1,3-propanediol fermentation from glycerol. In this report, gene gdh, encoding GDH of Klebsiella pneumoniae KG1 was gained by PCR with genomic DNA as template. The open reading frame (ORF) of gdh consisted of 1,143 bp and encoded 380 amino acids with a deduced molecular mass of 42 kDa. The gene gdh was overexpressed in E. coli BL21 (DE3) and produced 886-fold higher activity of GDH than that of KG1. The optimum temperature of recombinant GDH was 37 °C and glycerol was the optimum substrate.