Perfusion flow culture improves growth of osteogenic cells in a gradient calcium polyphosphate scaffold

摘要

The perfusion flow bioreactor was successfully developed. It improved the cell cultures. The gradient structure did affect the cellular behavior. The 1st and 2nd layers of the gradient scaffold have larger pore size and porosity which is beneficial for cellular attachment and growth. Compare to homogenous scaffold, although the gradient structure did not improved the cellular proliferation because the total space for cell growth was similar, it enhanced cellular differentiation significantly. Thus, the gradient structure impacted cellular distributions and differentiation which has potential value of clinical application.