Engineering a Biomimetic Periosteum for Cortical Bone Allografts: Incorporation of Heparin-Binding Growth Factors into Chitosan-Based Tissue Engineering Scaffolds

摘要

PEM-modified allografts demonstrate sustained release of FGF-2 over 14 days in vitro. Released FGF-2 can potentially recruit endogenous osteoprogenitor cells. With only 1.5% of total FGF-2 adsorbed released, there is a significant amount of FGF-2 left on our scaffolds which can provide highly localized signaling to osteoprogenitor cells. An average of 7.5 ng of FGF-2 adsorbed can also potentially provide an optimal mitogenic effect for osteoprogenitor cells. PEM-modified tissue engineering scaffolds provide a method for facile incorporation of heparin-binding growth factors. Release profiles of FGF-2 and other heparin-binding growth factors are tunable through the addition of more multilayers or addition of nanoparticles. Further investigation is warranted to assess the bioactivity of the growth factors adsorbed and released.